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plant nuclear protein  (Beijing Solarbio Science)


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    Beijing Solarbio Science plant nuclear protein
    Plant Nuclear Protein, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plant+nuclear+protein/pm40444705-56-8-16?v=Beijing+Solarbio+Science
    Average 93 stars, based on 3 article reviews
    plant nuclear protein - by Bioz Stars, 2026-08
    93/100 stars

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    (A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
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    (A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a cytosolic marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).

    Journal: bioRxiv

    Article Title: Phosphorus Availability Modulates Flowering Time Through Subcellular Reprogramming of bGLU25 and GRP7 in Flowering Plants

    doi: 10.1101/2025.01.02.631137

    Figure Lengend Snippet: (A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a cytosolic marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).

    Article Snippet: To separate cytosolic, ER-enriched, or nuclear fractions, Arabidopsis plants were homogenized and fractionated using either the Minute™ Plant Microsomal Membrane Extraction Kit (Invent Biotechnologies, MM-018) or the Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit (Invent Biotechnologies, PF-045), following the manufacturer’s instructions.

    Techniques: Bimolecular Fluorescence Complementation Assay, Expressing, Marker